脊髓损伤后大鼠后肢运动功能恢复不同评分标准比较.docVIP

脊髓损伤后大鼠后肢运动功能恢复不同评分标准比较 　【关键词】; 脊髓损伤；运动功能；评分标准 毕业论文 　　摘要：目的; 比较脊髓损伤(SCI)后大鼠后肢运动功能恢复的不同评分标准的优劣。方法; 40只SD成熟雌性大鼠随机分为正常组(Normal组)、急性脊髓中度损伤组(SCI组)及对照组(CON组)，其中SCI组采用改良的Allen打击法，CON组仅行T10椎板切除术。术后1、2、3、4、6周观察大鼠后肢神经功能恢复的情况并记录结果。评价标准分别为：斜板试验评分、改良Tarlov评分及BBB评分。结果; SCI组与CON组比较，斜板试验临界角度在1-6周时，均有所减小(Plt;0.05)，尤以第1周时减少更甚(Plt;0.01)；改良Tarlov评分第1、2、3、4周时，分值间的差别非常明显(Plt;0.01)，第6周时，未见变化(Pgt;0.05)；而BBB评分各时间点的区分程度非常明显(Plt;0.01)。结论; BBB 评分对SCI 模型运动功能评价具有明显优势，可作为今后研究的标准评分法。 毕业论文 　　关键词：脊髓损伤；运动功能；评分标准 毕业论文 　　A compare of hindlimbs locomotor function recovery　after spinal cord injury in rats 毕业论文 　　ABSTRACT: Objective; To contrast the different scale rule of hindlimbs locomotor function recovery after the rat spinal cord was injured. Methods; A total of 40 rats were divided into 3 groups: normal group, spinal cord injury group (SCI) (Modified Allen method), and control group (CON) (only T10 laminectomy). To observe nerve functional recovery of hindlimbs after operation in 1w, 2w, 3w, 4w, 6w respectively. Score standard: Inclined plane method, Modified Tarlov grading, BBB scale and recording results. Results; To contrast SCI group and control group, critical angle in incline plane test was decreased in 6 weeks(Plt;0.05), especially in 1 week (Plt;0.01); Modified Tarlov grading was compared: 1w, 2w, 3w, 4w, the difference of scale was greatly different (Plt;0.01), but in 6w this change disappeared (Pgt;0.05); in convalescence stage(from 1w to 6w), the degree of every one of BBB scales was distinguished(Plt;0.01). Conclusion; It is reasonable that the BBB scale is a standard test for SCI locomotor function assessment in rats. 毕业论文 　　KEY WORDS: spinal cord injury group (SCI); locomotor function; scale rule 毕业论文 　　建立脊髓损伤(spinal cord injury, SCI)后神经功能评定的方法，是确定SCI程度、观察恢复情况及评价药物的最基本要求。SCI后神经功能评价方法主要有神经电生理、组织形态和功能评定。由于动物对检测应答具有局限性，所以多年来一直侧重于SCI后运动功能的评估。 毕业论文 　　1; 材料与方法 毕业论文 　　1.1; 动物分组及模型的建立 毕业论文 　　1.1.1; 动物来源及分组; 成熟SD(SpragueDawley)雌性大鼠40只，体重250-300g，由西安交通大学医学院实验动物中心提供。随机分为急性中度脊髓