苗药八爪金龙半仿生提取工艺优选及其提取物抑菌活性探究.docVIP

苗药八爪金龙半仿生提取工艺优选及其提取物抑菌活性探究 　 作者：王瑞，刘晓燕，马立志，高蓬明，徐洪 【摘要】 目的通过均匀设计法优选八爪金龙半仿生提取工艺条件，并对其提取物的抑菌活性进行研究。方法以岩白菜素含量、HPLC总面积、干浸膏得率为指标，进行综合评判，并将3种工艺提取物的抑菌活性进行比较。结果最佳半仿生提取工艺为八爪金龙加8倍、8倍、6倍水提取3次，3次煎水pH值分别为2.0，6.5，9.0；提取时间依次为85，40，20 min。综合评价Y值及抑菌活性顺序为：醇提取法gt;半仿生提取法gt;水提法。结论优选出的半仿生工艺是可行的，且优于水提工艺。 【关键词】 八爪金龙；半仿生提取；工艺条件；抑菌活性 　　Abstract：ObjectiveTo obtain the best extraction condition of semi-bionic for Ardisia crenata Sims by uniform design and study its antibacterial activity.MethodsThe semi-bionic extraction (SBE) conditions were optimized by comprehensive evaluation with the content of bergenin, total HPLC integrate area and dried extract weight were adopted as markers. The antibacterial activities of extracts from three different processes were investigated. ResultsThe Ardisia crenata Sims was extracted in water 8, 8, 6 times as much as it for 3 times; the pH values of the water for the thrice extraction were 2.0, 6.5, 9.0; the extraction time were 85, 40, 20 min. The order of comprehensive evaluation value and antibacterial activities was as follows: AE, SBE, WE. ConclusionOptimization of SBE method of Ardisia crenata Sims by uniform design is available and better than WE method. 　　Key words：Ardisia crenata Sims; Semi-bionic extraction method; Process conditions; Antibacterial activities 　　八爪金龙，被苗族奉为喉科良药，为紫金牛科植物朱砂根Ardisia crenata Sims的干燥根及根茎, 具清热解毒、散瘀止痛、祛风除湿之功能, 用于治疗咽喉肿痛、扁桃体炎、心胃气痛、劳伤吐血、跌扑损伤、风湿骨痛［1］。该药材在贵州省分布广、蕴藏量大，为贵州民间习用中药。有关文献报道，该药材提取物—岩白菜素及其衍生物对白血细胞、肺癌细胞、口腔上皮癌细胞、宫颈瘤、肝H22肿瘤等具有明显抑制作用［2］及良好的抗HIV病毒作用［3］；所含三萜总皂苷具有抑制血小板凝聚作用［4］；环苯缩酚酸肽具有降低血压作用［5］。 半仿生提取法(Semi-bionic Extraction method，简称SBE法)已在单方和复方制剂工艺中得到一定应用，其结果表明该技术符合口服药物经胃肠道转运吸收的原理，该工艺提取物更接近药物在体内达到平衡后的有效成分群，有可能替代传统的中药提取技术［6］。本研究通过均匀设计法，以岩白菜素含量、HPLC总面积、干浸膏得率为综合评判指标，优选出八爪金龙SBE工艺条件，并将其SBE工艺产物与醇提法产物、水提法产物进行综合评判指标及抑菌活性对比。 　　1 仪器与材料 高效液相色谱仪(Agilent 1100高效液相色谱仪、二极管阵列检测器、原装色谱工作站)；色谱柱：DiamonsilTM(钻石)C18 (250 mm×4.6 mm, 5 μm)；PHS2-3C型精密pH计(上海雷磁仪器厂)；AUW220D电子分析天平(岛津)；RE-52A型旋转蒸发仪(上海亚荣)；ES-315 TOMY高压蒸气灭菌锅(基因公司)；BCN-1360B生物洁净工作台(广东半球仪器厂)；