蒙药材草乌及其炮制品中几种双酯型生物碱含量比较.docVIP

蒙药材草乌及其炮制品中几种双酯型生物碱含量比较 　 作者：吕颖 王栋，林燕，王伟，孙立新 【摘要】 　　目的用高效液相色谱法比较蒙药材草乌及其炮制品中乌头碱、次乌头碱、新乌头碱的含量。方法色谱柱为Diamonsil C18柱（250 mm×4.6 mm,5 μm）；流动相A为乙腈-四氢呋喃（25∶15, V/V），B为 0.1 mol·L-1醋酸铵-冰醋酸(100∶0.05,V/V)，梯度洗脱；检测波长为235 nm；流速1.0 ml·min-1；柱温为30℃；进样量10 μl。结果蒙药材草乌经特殊炮制后，炮制品中乌头碱、次乌头碱、新乌头碱的含量均低于草乌生品，诃子制草乌中含量最低，甘草制草乌含量次之，酸奶制草乌含量最高。结论3种炮制方法使草乌药材中双酯型生物碱含量大大降低，可用于指导蒙医临床用药。 【关键词】 草乌；炮制品；高效液相色谱法；乌头碱；次乌头碱； 新乌头碱 　　Abstract:ObjectiveTo compare the contents of aconitine，hypaconitine and mesaconitine in Radix Aconiti Kusnezoffii and its mongolian distinct preparations by HPLC.MethodsDiamonsil C18(250 mm×4.6 mm,5 μm) was used as chromatographic column, acetonitrile-tetrahydrofuran(25∶15,V/V) and 0.1 mol·L-1 ammonium acetate - acetic acid (100∶0.05,V/V) in gradient elution mode as mobile phase. The flow rate was set at 1.0 ml·min-1, the detection wavelength at 235 nm and the column temperature was at 30℃.ResultsAfter Radix Aconiti Kusnezoffii was processed by Mongolian distinct technology, the contents of aconitine, hypaconine and mesaconitine in its preparations were less than those in unprocessed Radix Aconiti. Kusnezoffii, and in these preparations the total content of three alkaloids thin myrobalan preparation of Radix Aconiti Kusnezoffii was minimum,that in liquorice preparation of Radix Aconiti Kusnezoffii was second,and that in acidophilous milk preparation of Radix Aconiti Kusnezoffii was maximum.ConclusionThe contents of diesters alkaloid in Radix Aconiti Kusnezoffii processed by three Mongolian distinct technology decrease largely, which can help to guide traditional Mongolian clinical medication. 　　Key words:Radix Aconiti Kusnezoffii； Mongolian distinct preparations； HPLC； Aconitine； Hypaconitine； Mesaconitine 　　草乌为毛茛科植物北乌头Aconitum kusnezo ffii Reichb.的干燥块根，具有祛风除湿、温经止痛功能［1］，为蒙医临床常用药物之一。草乌主要成分是生物碱，生物碱既是其生理活性物质，也是毒性物质，尤其是二萜双酯型生物碱毒性最强，如乌头碱、次乌头碱、新乌头碱［2］。其用药剂量与中毒剂量接近，经炮制加工后，毒性大大降低。因此，炮制过程中应严格控制双酯型生物碱的转化程度，防止炮制不充分，有毒成分含量偏高；炮制太过，有效成分流失严重，影响药效。不同蒙药炮制方法对草乌中所含双酯型生物碱含量的影响报道较少，为保证蒙医用药安全，比较草乌药材