a novel cell culture model for studying differentiation and apoptosis in the mouse mammary gland一种新型细胞培养模型研究分化和细胞凋亡在鼠标乳腺.pdfVIP

/content/2/3/222 Primary research A novel cell culture model for studying differentiation and apoptosis in the mouse mammary gland † † Katrina E Gordon, Bert Binas*, Rachel S Chapman , Kathreena M Kurian , ‡ § ‡ Richard W E Clarkson , A John Clark, E Birgitte Lane and Christine J Watson Roslin Institute (Edinburgh), Roslin, UK, *Max Delbruck Center for Molecular Medicine, Berlin, Germany, †University of Edinburgh, Edinburgh, UK, ‡University of Cambridge, Cambridge, UK, and §University of Dundee, Dundee, UK Received: 17 August 1999 Breast Cancer Res 2000, 2:222–235 Revisions requested: 12 October 1999 The electronic version of this article can be found online at Revisions received: 28 January 2000 /content/2/3/222 Accepted: 8 February 2000 Published: 27 March 2000 © Current Science Ltd Abstract Background: This paper describes the derivation and characterization of a novel, conditionally immortal mammary epithelial cell line named KIM-2. These cells were derived from mid-pregnant mammary glands of a mouse harbouring one to two copies of a transgene comprised of the ovine -lactoglobulin milk protein gene promoter, driving expression of a temperature-sensitive variant of simian virus-40 (SV40) large T antigen (T-Ag). Results: KIM-2 cells have a c