a stable explant culture of her2neu invasive carcinoma supported by alpha-smooth muscle actin expressing stromal cells to evaluate therapeutic agents稳定的her2neu外植体培养alpha-smooth肌肉肌动蛋白表达支持的浸润性癌基质细胞评估治疗药物.pdfVIP

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a stable explant culture of her2neu invasive carcinoma supported by alpha-smooth muscle actin expressing stromal cells to evaluate therapeutic agents稳定的her2neu外植体培养alpha-smooth肌肉肌动蛋白表达支持的浸润性癌基质细胞评估治疗药物.pdf

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a stable explant culture of her2neu invasive carcinoma supported by alpha-smooth muscle actin expressing stromal cells to evaluate therapeutic agents稳定的her2neu外植体培养alpha-smooth肌肉肌动蛋白表达支持的浸润性癌基质细胞评估治疗药物

BMC Cancer BioMed Central Research article Open Access A stable explant culture of HER2/neu invasive carcinoma supported by alpha-Smooth Muscle Actin expressing stromal cells to evaluate therapeutic agents Marie P Piechocki Address: Department of Breast Cancer Immunotherapy, Wayne State University and Karmanos Cancer Center, Detroit, MI, USA Email: Marie P Piechocki - piechock@ Published: 24 April 2008 Received: 24 October 2007 Accepted: 24 April 2008 BMC Cancer 2008, 8:119 doi:10.1186/1471-2407-8-119 This article is available from: /1471-2407/8/119 © 2008 Piechocki; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: To gain a better understanding of the effects of therapeutic agents on the tumor microenvironment in invasive cancers, we developed a co-culture model from an invasive lobular carcinoma. Tumor cells expressing HER2/neu organize in nests surrounded by alpha-Smooth Muscle Actin (α-SMA) expressing tumor stroma to resemble the morphology of an invading tumor. This co-culture, Mammary Adenocarcinoma Model (MAM-1) maintains a 1:1 ratio of HER2/neu positive tumor cells to α-SMA-reactive stromal cells and renews this configuration for over 20 passages in vitro. Methods: We chara