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advancing eucalyptus genomics identification and sequencing of lignin biosynthesis genes from deep-coverage bac libraries促进桉树基因识别和木质素生物合成基因的测序deep-coverage bac库
Paiva et al. BMC Genomics 2011, 12:137
/1471-2164/12/137
RESEARCH ARTICLE Open Access
Advancing Eucalyptus genomics: identification
and sequencing of lignin biosynthesis genes from
deep-coverage BAC libraries
1,2* 3 3 4 5,6 7
Jorge AP Paiva , Elisa Prat , Sonia Vautrin , Mauro D Santos , Hélène San-Clemente , Sérgio Brommonschenkel ,
4 8 9 9 9 9
Paulo GS Fonseca , Dario Grattapaglia , Xiang Song , Jetty SS Ammiraju , David Kudrna , Rod A Wing ,
4 3 5,6
Ana T Freitas , Hélène Bergès , Jacqueline Grima-Pettenati
Abstract
Background: Eucalyptus species are among the most planted hardwoods in the world because of their rapid
growth, adaptability and valuable wood properties. The development and integration of genomic resources into
breeding practice will be increasingly important in the decades to come. Bacterial artificial chromosome (BAC)
libraries are key genomic tools that enable positional cloning of important traits, synteny evaluation, and the
development of genome framework physical maps for genetic linkage and genome sequencing.
Results: We describe the construction and characterization of two deep-coverage BAC libraries EG_Ba and EG_Bb
obtained from nuclear DNA fragments of E. grandis (clone BRASUZ1) digested with HindIII and BstYI, respectively.
Genome coverages of 17 and 15 haploid genome equivalents were estimated for EG_Ba and EG_Bb, respectively. Both
libraries contained large inserts, with average sizes ranging from 135 Kb (Eg_Bb) to 157 Kb (Eg_Ba), very low extra-nuclear
genome contamina
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