an in silico approach combined with in vivo experiments enables the identification of a new protein whose overexpression can compensate for specific respiratory defects in saccharomyces cerevisiae在硅片的方法结合体内实验能够识别新的蛋白质的过度可以弥补特定的呼吸缺陷在酿酒酵母.pdfVIP

Glatigny et al. BMC Systems Biology 2011, 5:173 /1752-0509/5/173 RESEARCH ARTICLE Open Access An in silico approach combined with in vivo experiments enables the identification of a new protein whose overexpression can compensate for specific respiratory defects in Saccharomyces cerevisiae 1* 2 1 1 1 Annie Glatigny , Lise Mathieu , Christopher J Herbert , Geneviève Dujardin , Brigitte Meunier and Marie-Hélène Mucchielli-Giorgi1,3 Abstract Background: The mitochondrial inner membrane contains five large complexes that are essential for oxidative phosphorylation. Although the structure and the catalytic mechanisms of the respiratory complexes have been progressively established, their biogenesis is far from being fully understood. Very few complex III assembly factors have been identified so far. It is probable that more factors are needed for the assembly of a functional complex, but that the genetic approaches used to date have not been able to identify them. We have developed a systems biology approach to identify new factors controlling complex III biogenesis. Results: We collected all the physical protein-protein interactions (PPI) involving the core subunits, the supernumerary subunits and the assembly factors of complex III and used Cytoscape 2.6.3 and its plugins to construct a network. It was then divided into overlapping and highly interconnected sub-graphs with clusterONE. One sub-graph contained the core and the supernumerary subunits of complex III, it also contained some subunits of complex IV and proteins participating in the assembly of complex IV. This sub-graph was then split with another algorithm into two sub-graphs. The subtraction of these two sub-graphs from the pre