antioxidant activity relationship of phenolic compounds in hypericum perforatum l.贯叶连翘中酚类化合物的抗氧化活性的关系。.pdfVIP

Orčić et al. Chemistry Central Journal 2011, 5:34 /content/5/1/34 RESEARCH ARTICLE Open Access Antioxidant activity relationship of phenolic compounds in Hypericum perforatum L. 1 1* 1 2 1 Dejan Z Orčić , Neda M Mimica-Dukić , Marina M Francišković , Slobodan S Petrović and Emilija Đ Jovin Abstract Background: The St John’s Wort (Hypericum perforatum; Clusiaceae) has been used in traditional and modern medicine for a long time due to its high content of biologically active phenolics. The purpose of this work was to develop a method for their fractionation and identification, and to determine the most active antioxidant compounds in plant extract. Results: An LC-MS method which enables fast qualitative and semiquantitative analysis was developed. The composition determined is in agreement with the previous results, where 6 flavonoids, 4 naphthodianthrones and 4 phloroglucinols have been identified. Significant antioxidant activity was determined for most of the fractions by DPPH assay (the lowest IC50 of 0.52 μg/ml), NO scavenging (6.11 μg/ml), superoxide scavenging (1.86 μg/ml), lipid peroxidation (0.0079 μg/ml) and FRAP (the highest reduction capacity of 104 mg Fe equivalents/g) assays. Conclusion: LC-MS technique has been successfully applied for a quick separation and identification of the major components of H. perforatum fractions. Majority of the fractions analyzed have expressed a very high antioxidative activity when compared to synthetic antioxidants. The antioxidant activity could be attributed to flavonoids and phenolic acids, while phloroglucinols and naphthodianthrones showed no significant activity. It is demonstrated that it is possible to obtain, by