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apm_gui analyzing particle movement on the cell membrane and determining confinementapm_gui分析粒子运动在细胞膜和确定监禁
Menchón et al. BMC Biophysics 2012, 5:4
/2046-1682/5/4
SOFTWARE Open Access
APM_GUI: analyzing particle movement on the
cell membrane and determining confinement
Silvia A Menchón1,2*, Mauricio G Martín1,3 and Carlos G Dotti1,3*
Abstract
Background: Single-particle tracking is a powerful tool for tracking individual particles with high precision. It
provides useful information that allows the study of diffusion properties as well as the dynamics of movement.
Changes in particle movement behavior, such as transitions between Brownian motion and temporary
confinement, can reveal interesting biophysical interactions. Although useful applications exist to determine the
paths of individual particles, only a few software implementations are available to analyze these data, and these
implementations are generally not user-friendly and do not have a graphical interface,.
Results: Here, we present APM_GUI (Analyzing Particle Movement), which is a MatLab-implemented application
with a Graphical User Interface. This user-friendly application detects confined movement considering non-random
confinement when a particle remains in a region longer than a Brownian diffusant would remain. In addition,
APM_GUI exports the results, which allows users to analyze this information using software that they are familiar
with.
Conclusions: APM_GUI provides an open-source tool that quantifies diffusion coefficients and determines whether
trajectories have non-random confinements. It also offers a simple and user-friendly tool that can be used by
individuals without programming skills.
Background and open-source tool for the analysis of data from two-
Informatics tools have become essential for biologists dimensional single-particle tracking (SPT) experi
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