association between helicobacter pylori caga-related genes and clinical outcomes in colombia and japan幽门螺杆菌caga-related基因和临床结果之间的联系在哥伦比亚和日本.pdfVIP

Watada et al. BMC Gastroenterology 2011, 11:141 /1471-230X/11/141 RESEARCH ARTICLE Open Access Association between Helicobacter pylori cagA- related genes and clinical outcomes in Colombia and Japan 1,2 1,2 1,2 1 2 2 Masahide Watada , Seiji Shiota , Osamu Matsunari , Rumiko Suzuki , Kazunari Murakami , Toshio Fujioka and Yoshio Yamaoka1,3* Abstract Background: Specific genotypes of several virulence factors of Helicobacter pylori (eg, cagA-positive, vacA s1, oipA “on” and babA-positive) have been reported to be predictors of severe clinical outcomes. Importantly, the presence of these genotypes correlates with each other. We hypothesized that novel virulence genes correlate with the presence of cagA. Therefore, we aimed to find novel candidate virulence genes that correlate with cagA and examined the association of these genes with clinical outcomes in Colombian and Japanese populations. Methods: cagA-associated genes were selected based on previous H. pylori genome microarray data. A total of 343 strains (174 from Colombia and 169 from Japan) were examined for the status of cagA, vacA, and candidate genes by polymerase chain reaction and dot blot. Results: Microarray data showed that 9 genes were significantly correlated with the presence of cagA. Among the 9 genes, the functions of 4 were known, and we selected these 4 genes as candidate genes (hp0967, jhp0045, jhp0046 , and jhp0951 ). The prevalences of cagA, vacA s1/m1 genotype, and hp0967 were significantly higher in Japan than Colombia, whereas those of jhp0045 and jhp0046 were more prevalent in Colombia than Japan. The prevalences of jhp0045 and jhp0046 in cagA-positive cases of gastric cancer were si