atrtprimer database for arabidopsis genome-wide homogeneous and specific rt-pcr primer-pairsatrtprimer数据库拟南芥全基因组均匀和特定的rt - pcr primer-pairs.pdfVIP
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atrtprimer database for arabidopsis genome-wide homogeneous and specific rt-pcr primer-pairsatrtprimer数据库拟南芥全基因组均匀和特定的rt - pcr primer-pairs
BMC Bioinformatics BioMed Central
Database Open Access
AtRTPrimer: database for Arabidopsis genome-wide homogeneous
and specific RT-PCR primer-pairs
Sangjo Han and Dongsup Kim*
Address: Department of BioSystems, Korea Advanced Institute of Science and Technology, 373-1 Guseong-dong, Yuseong-gu, Daejeon 305-701,
Republic of Korea
Email: Sangjo Han - Sangjo_Han@kaist.ac.kr; Dongsup Kim* - kds@kaist.ac.kr
* Corresponding author
Published: 30 March 2006 Received: 07 October 2005
Accepted: 30 March 2006
BMC Bioinformatics 2006, 7:179 doi:10.1186/1471-2105-7-179
This article is available from: /1471-2105/7/179
© 2006 Han and Kim; licensee BioMed Central Ltd.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0),
which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Background: Primer design is a critical step in all types of RT-PCR methods to ensure specificity
and efficiency of a target amplicon. However, most traditional primer design programs suggest
primers on a single template of limited genetic complexity. To provide researchers with a sufficient
number of pre-designed specific RT-PCR primer pairs for whole genes in Arabidopsis, we aimed to
construct a genome-wide primer-pair database.
Description: We considered the homogeneous physical and chemical properties of each primer
(homogeneity) of a gene, non-specific b
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