high-throughput sequencing of black pepper root transcriptome转录组高通量测序的黑胡椒根.pdfVIP

Gordo et al. BMC Plant Biology 2012, 12:168 /1471-2229/12/168 RESEARCH ARTICLE Open Access High-throughput sequencing of black pepper root transcriptome 1 2 1 3 3 Sheila MC Gordo , Daniel G Pinheiro , Edith CO Moreira , Simone M Rodrigues , Marli C Poltronieri , 3 2 4 4 1 2 Oriel F de Lemos , Israel Tojal da Silva , Rommel TJ Ramos , Artur Silva , Horacio Schneider , Wilson A Silva Jr , Iracilda Sampaio1 and Sylvain Darnet4* Abstract Background: Black pepper (Piper nigrum L.) is one of the most popular spices in the world. It is used in cooking and the preservation of food and even has medicinal properties. Losses in production from disease are a major limitation in the culture of this crop. The major diseases are root rot and foot rot, which are results of root infection by Fusarium solani and Phytophtora capsici, respectively. Understanding the molecular interaction between the pathogens and the host’s root region is important for obtaining resistant cultivars by biotechnological breeding. Genetic and molecular data for this species, though, are limited. In this paper, RNA-Seq technology has been employed, for the first time, to describe the root transcriptome of black pepper. Results: The root transcriptome of black pepper was sequenced by the NGS SOLiD platform and assembled using the multiple-k method. Blast2Go and orthoMCL methods were used to annotate 10338 unigenes. The 4472 predicted proteins showed about 52% homology with the Arabidopsis proteome. Two root proteomes identified 615 proteins, which seem to define the plant’s root pattern. Simple-sequence repeats were id