hitherto unrecognized fluorescence properties of coniferyl alcohol迄今为止未被松柏醇的荧光特性.pdfVIP

Molecules 2010, 15, 1645-1667; doi:10.3390/molecule OPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Hitherto Unrecognized Fluorescence Properties of Coniferyl Alcohol Komandoor Elayavalli Achyuthan 1,2,*, Paul David Adams 1,3, Supratim Datta 1,4, Blake Alexander Simmons 1,4 and Anup Kumar Singh 1,4 1 Joint BioEnergy Institute (JBEI), Emeryville, CA 94550, USA 2 Biosensors and Nanomaterials Department, Sandia National Laboratories, Albuquerque, NM 87185, USA 3 Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA; E-Mail: pdadams@ (P.D.A.) 4 Sandia National Laboratories, Livermore, CA 94550, USA; E-Mails: basimmo@ (B.A.S.); sdatta2@ (S.D.); aksingh@ (A.K.S.)  Author to whom correspondence should be addressed; E-Mails: kachyut@ or keachyuthan@; Tel.: +1-505-284-8979; Fax: +1-505-844-1198. Received: 14 January 2010; in revised form: 23 February 2010 / Accepted: 8 March 2010 / Published: 11 March 2010 Abstract: We instituted a quasi-quality assurance program for demonstrating coniferyl alcohol’s fluorescence and fluorescence diminishment following enzymatic oxidation. The magnitude of diminishment was a measure of catalysis. High throughput screening was performed in pseudo -kinetic and endpoint modes by measuring the fluorescence at 416 nm following excitation at 290, 310 or 340 nm. Dose-response tracings were linear between two and three orders of magnitude with average limits of detection and quantitation of 1.8 and 6.9 M coniferyl alcohol, respectively. Oxidation was e