evaluation of the antinociceptive and anti-inflammatory effects of essential oil of nepeta pogonosperma jamzad et assadi in rats评价antinociceptive和抗炎效果的精油的荆芥pogonosperma jamzad et assadi老鼠.pdfVIP

Ali et al. DARU Journal of Pharmaceutical Sciences 2012, 20:48 /content/20/1/48 RESEARCH ARTICLE Open Access Evaluation of the antinociceptive and anti-inflammatory effects of essential oil of Nepeta pogonosperma Jamzad et Assadi in rats 1,2* 2 3 2 Taskina Ali , Mohammad Javan , Ali Sonboli and Saeed Semnanian Abstract Background and the purpose of study: Concerning the different effects of essential oils from Nepeta genus on the central nervous system including pain killing effect, this study was designed to evaluate the antinociceptive and anti-inflammatory effects of essential oil of Nepeta pogonosperma Jamzad et Assadi (NP), a recently identified species. Methods: Air-dried aerial parts of NP were hydrodistillated and GC-MS analysis of obtained essential oil was conducted. Total 24 male Wister rats weighing 225 ± 25 gm were studied. Essential oil of NP was administered intraperitoneally at the doses of 50 mg/kg, 100 mg/kg and 200 mg/kg for the experimental groups. Control rats received equal volume (2 ml/kg) of normal saline. Antinociception was assessed by tail flick test (after 30 minutes) and formalin test (for further 60 minutes). Then the animal was sacrificed and the paw edema was measured using a water plethysmometer. Results: 4aα,7α,7aβ-nepetalactone and 1,8-cineole were found as the main concentrated components of NP essential oil. All the doses of NP showed antinociception. NP 200 mg/kg reduced the pain sensation in tail flick (p 0.01) and formalin test (p 0.001 in both phases). In paw edema test, NP 100 and 200 mg/kg significantly reduced the inflammation (p 0.01 and p 0.05). Conclusion: This study reveals that the essential oil of NP may minimize both the acute and chronic forms of nociceptio