interest of fluorescence derivatization and fluorescence probe assisted post-column detection of phospholipids a short review荧光衍生化和荧光探针协助邮报的专栏里检测磷脂的一个简短的评论.pdfVIP

Molecules 2010, 15, 352-373; doi:10.3390/molecule OPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Review Interest of Fluorescence Derivatization and Fluorescence Probe Assisted Post-column Detection of Phospholipids: A Short Review Hanadi Ibrahim, Eric Caudron *, Athena Kasselouri and Pratrice Prognon Groupe de Chimie Analytique de Paris-Sud, Faculty of Pharmacy, University Paris-Sud, EA 4041, IFR 141, 92296 Châtenay-Malabry, France * Author to whom correspondence should be addressed; E-Mail: eric.caudron@u-psud.fr. Received: 2 December 2009; in revised form: 9 January 2010 / Accepted: 12 January 2010 / Published: 18 January 2010 Abstract: Phospholipids are essential constituents of all living cell membranes. There are many analytical methods available for the quantitative and qualitative determination of phospholipids, but since these molecules lack chromophores, common absorbance based methods are of limited use. Beside mass spectrometry, some less specific approaches that are routinely used are evaporative light scattering detection or fluorescence, which exhibit sufficient sensitivity. Here, we focus on fluorescence, which remains an interesting way to quantify phospholipids. Two ways of detecting phospholipids by fluorescence are possible coupled with separation techniques such as thin layer chromatography (TLC), high performance liquid chromatography (HPLC) and capillary electrophoresis (CE): firstly, pre-column derivatization procedures and s