mucosa-associated but not luminal escherichia coli is augmented in crohn’s disease and ulcerative colitismucosa-associated但不是腔的大肠杆菌在克罗恩病和溃疡性结肠炎增强.pdfVIP

de Souza et al. Gut Pathogens 2012, 4:21 /content/4/1/21 RESEARCH Open Access Mucosa-associated but not luminal Escherichia coli is augmented in Crohn’s disease and ulcerative colitis 1 1 2 2 Helton Luis de Souza , Vanessa R de Carvalho , Fernando Gomes Romeiro , Ligia Yukie Sassaki , Rogeria Keller1 and Josias Rodrigues1* Abstract Background: Escherichia coli is believed to participate in the etiology of Crohn’s disease (CD) and possibly of ulcerative colitis (UC), due at least in part to the observed rise in the number of these bacteria in the gut microbiota of CD and UC patients. Nevertheless, it is not fully understood whether this quantitative variation occurs equally throughout the mucosal and luminal spaces of the gut. To assess this question, stools and mucosa biopsies from distinct intestinal sites were cultured aiming at determining their E. coli concentration. The cultures were additionally screened for the presence of some virulence genes of pathogenic E. coli. Results: Analyses of clinical materials from 14 controls (38 biopsies and 14 stools samples), 11 CD (25 biopsies and 11 stools samples) and 7 UC patients (18 biopsies and 7 stools samples) indicated no significant variation in the number of E. coli present in stools, but a rise of at least one log10 CFU/mg in biopsies from the ileum of CD patients and the sigmoid and rectum of CD and UC patients. The cultures were screened for the presence of E. coli attaching and effacing (eae), invasion plasmid antigen H (ipaH), aggregative adherence transcriptional activator (aggR), Shiga cytotoxins (stx), and heat labile enterotoxin (elt) and the following serine proteases autotransporters of Enterobacteriaceae (SPATE) genes: plasmid e