nature of fatty acids in high fat diets differentially delineates obesity-linked metabolic syndrome components in male and female c57bl6j mice高脂肪饮食中脂肪酸的不同划定obesity-linked代谢综合征组件在雄性和雌性c57bl6j老鼠.pdfVIP

El Akoum et al. Diabetology Metabolic Syndrome 2011, 3:34 DIABETOLOGY /content/3/1/34 METABOLIC SYNDROME RESEARCH Open Access Nature of fatty acids in high fat diets differentially delineates obesity-linked metabolic syndrome components in male and female C57BL/6J mice Souhad El Akoum1,2, Vikie Lamontagne1,2, Isabelle Cloutier1 and Jean-François Tanguay1,2* Abstract Background: Adverse effects of high-fat diets (HFD) on metabolic homeostasis are linked to adipose tissue dysfunction. The goal of this study was to examine the effect of the HFD nature on adipose tissue activity, metabolic disturbances and glucose homeostasis alterations in male mice compared with female mice. Methods: C57BL/6J mice were fed either a chow diet or HFD including vegetal (VD) or animal (AD) fat. Body weight, plasmatic parameters and adipose tissue mRNA expression levels of key genes were evaluated after 20 weeks of HFD feeding. Results: HFD-fed mice were significantly heavier than control at the end of the protocol. Greater abdominal visceral fat accumulation was observed in mice fed with AD compared to those fed a chow diet or VD. Correlated with weight gain, leptin levels in systemic circulation were increased in HFD-fed mice in both sexes with a significant higher level in AD group compared to VD group. Circulating adiponectin levels as well as adipose tissue mRNA expression levels were significantly decreased in HFD-fed male mice. Although its plasma levels remained unchanged in females, adiponectin mRNA levels were significantly reduced in adipose tissue of both HFD-fed groups with a more marked decrease in AD group compare