new investigations around cyp11a1 and its possible involvement in an androstenone qtl characterised in large white pigs新调查cyp11a1特征和其可能参与一个雄烯酮qtl在白色大猪.pdfVIP

Robic et al. Genetics Selection Evolution 2011, 43:15 Ge n e t i c s /content/43/1/15 Se l e c t i o n Evolution RESEARCH Open Access New investigations around CYP11A1 and its possible involvement in an androstenone QTL characterised in Large White pigs 1* 2 1 2 1 Annie Robic , Guillaume Le Mignon , Katia Fève , Catherine Larzul and Juliette Riquet Abstract Background: Previously, in boars with extreme androstenone levels, differential expression of the CYP11A1 gene in the testes has been characterised. CYP11A1 is located in a region where a QTL influencing boar fat androstenone levels has been detected in a Large White pig population. Clarifying the role of CYP11A1 in boar taint is important because it catalyses the initial step of androstenone synthesis and also of steroid synthesis. Results: A genome-wide association study located CYP11A1 at approximately 1300 kb upstream from SNP H3GA0021967, defining the centre of the region containing the QTL for androstenone variation. In this study, we partially sequenced the CYP11A1 gene and identified several new single nucleotide polymorphisms (SNP) within it. Characterisation of one animal, heterozygous for CYP11A1 testicular expression but homozygous for a haplotype of a large region containing CYP11A1, revealed that variation of CYP11A1 expression is probably regulated by a mutation located downstream from the SNP H3GA0021967. We analysed CYP11A1 expression in LW families according to haplot