oligonucleotide-templated reactions for sensing nucleic acidsoligonucleotide-templated传感核酸的反应.pdfVIP

Molecules 2012, 17, 2446-2463; doi:10.3390/molecule OPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Review Oligonucleotide-Templated Reactions for Sensing Nucleic Acids Aya Shibata, Hiroshi Abe * and Yoshihiro Ito Nano Medical Engineering Laboratory, RIKEN Advanced Science Institute 2-1, Hirosawa, Wako-Shi, Saitama 351-0198, Japan; E-Mails: ashibata@riken.jp (A.S.); y-ito@riken.jp (Y.I.) * Author to whom correspondence should be addressed; E-Mail: h-abe@riken.jp; Tel.: +81-48-467-5749; Fax: +81-48-467-2827. Received: 10 February 2012; in revised form: 23 February 2012 / Accepted: 24 February 2012 / Published: 29 February 2012 Abstract: Oligonucleotide-templated reactions are useful for applying nucleic acid sensing. Various chemistries for oligonucleotide-templated reaction have been reported so far. Major scientific interests are focused on the development of signal amplification systems and signal generation systems. We introduce the recent advances of oligonucleotide-templated reaction in consideration of the above two points. Keywords: detection; amplification; fluorescence; DNA; protein nucleic acid; RNA 1. Introduction Oligonucleotide-templated reactions (OTRs) are powerful tools for the detection of nucleic acid sequences [1,2]. Basically, two probes that carry a reactive group bind to the template strand located in next each other and a chemical reaction proceeds on the template without any enzyme or additive reagent. This reaction ca