phage display of combinatorial peptide libraries application to antiviral research组合的噬菌体展示肽库应用抗病毒药物研究.pdfVIP

Molecules 2011, 16, 3499-3518; doi:10.3390/molecule OPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Review Phage Display of Combinatorial Peptide Libraries: Application to Antiviral Research Guillaume Castel, Mohamed Chtéoui, Bernadette Heyd and Noël Tordo * Unité Postulante des Stratégies Antivirales, CNRS URA-3015, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris Cedex 15, France * Author to whom correspondence should be addressed; E-Mail: ntordo@pasteur.fr; Tel.: +33 1 4061 3134; Fax: +33 1 4061 3256. Received: 12 March 2011; in revised form: 21 April 2011 / Accepted: 22 April 2011 / Published: 26 April 2011 Abstract: Given the growing number of diseases caused by emerging or endemic viruses, original strategies are urgently required: (1) for the identification of new drugs active against new viruses and (2) to deal with viral mutants in which resistance to existing antiviral molecules has been selected. In this context, antiviral peptides constitute a promising area for disease prevention and treatment. The identification and development of these inhibitory peptides require the high-throughput screening of combinatorial libraries. Phage-display is a powerful technique for selecting unique molecules with selective affinity for a specific target from highly diverse combinatorial libraries. In the last 15 years, the use of this technique for antiviral purposes and