原创力文档ph-based detection of phenylalnine by potentiometric and colorimetric methods的phenylalnine ph-based检测电位和比色方法.pdfVIP
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ph-based detection of phenylalnine by potentiometric and colorimetric methods的phenylalnine ph-based检测电位和比色方法
Sensors 2006, 6, 428-434
sensors
ISSN 1424-8220
© 2006 by MDPI
/sensors
pH-based Detection of Phenylalnine by Potentiometric and
Colorimetric Methods
*
Dorota G. Pijanowska and Elżbieta Remiszewska
Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences, ul. Ks. Trojdena
4, 02-109 Warsaw, Poland
*
Author to whom correspondence should be addressed. E-mail: depijano@ibib.waw.pl
Received: 1 September 2006 / Accepted: 4 January 2006 / Published: 7 April 2006
Abstract: In this paper, methods of sample preparation for potentiometric measurement of
phenylalanine are presented. Basing on the spectrophotometric measurements of
phenylalanine, the concentrations of reagents of the enzymatic reaction (10 mM L-Phe,
+
0,4 mM NAD , 2U L-PheDH) were determined. Then, the absorption spectrum of the
reaction product, NADH, was monitored (maximum peak at 340 nm). The results obtained
by the spectrophotometric method were compared with the results obtained by the
colourimetry, using pH indicators. The above-mentioned two methods will be used as
references for potentiometric measurements of phenylalanine concentration.
Keywords: Phenylalanine determination, enzymatic methods, pH-based biosensing.
Sensors 2006, 6 429
Introduction
Phenylalanine, an e
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