ph-based detection of phenylalnine by potentiometric and colorimetric methods的phenylalnine ph-based检测电位和比色方法.pdfVIP

Sensors 2006, 6, 428-434 sensors ISSN 1424-8220 © 2006 by MDPI /sensors pH-based Detection of Phenylalnine by Potentiometric and Colorimetric Methods * Dorota G. Pijanowska and Elżbieta Remiszewska Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences, ul. Ks. Trojdena 4, 02-109 Warsaw, Poland * Author to whom correspondence should be addressed. E-mail: depijano@ibib.waw.pl Received: 1 September 2006 / Accepted: 4 January 2006 / Published: 7 April 2006 Abstract: In this paper, methods of sample preparation for potentiometric measurement of phenylalanine are presented. Basing on the spectrophotometric measurements of phenylalanine, the concentrations of reagents of the enzymatic reaction (10 mM L-Phe, + 0,4 mM NAD , 2U L-PheDH) were determined. Then, the absorption spectrum of the reaction product, NADH, was monitored (maximum peak at 340 nm). The results obtained by the spectrophotometric method were compared with the results obtained by the colourimetry, using pH indicators. The above-mentioned two methods will be used as references for potentiometric measurements of phenylalanine concentration. Keywords: Phenylalanine determination, enzymatic methods, pH-based biosensing. Sensors 2006, 6 429 Introduction Phenylalanine, an e