phenotypic and functional abnormalities of bone marrow-derived dendritic cells in systemic lupus erythematosus骨骨髓来源树突状细胞的表型和功能异常的系统性红斑狼疮.pdfVIP

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phenotypic and functional abnormalities of bone marrow-derived dendritic cells in systemic lupus erythematosus骨骨髓来源树突状细胞的表型和功能异常的系统性红斑狼疮.pdf

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phenotypic and functional abnormalities of bone marrow-derived dendritic cells in systemic lupus erythematosus骨骨髓来源树突状细胞的表型和功能异常的系统性红斑狼疮

Nie et al. Arthritis Research Therapy 2010, 12:R91 /content/12/3/R91 R E S E A R C H A R T I C L E Open Access Research article Phenotypic and functional abnormalities of bone marrow-derived dendritic cells in systemic lupus erythematosus 1 1 2 1 1 1 1 Ying J Nie , Mo Y Mok , Godfrey CF Chan , Albert W Chan , Ou Jin , Sushma Kavikondala , Albert KW Lie and Chak S Lau*1 Abstract Introduction: Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by autoreactive T and B cells, which are believed to be secondary to deficient dendritic cells (DCs). However, whether DC abnormalities occur during their development in the bone marrow (BM) or in the periphery is not known. Methods: Thirteen patients with SLE and 16 normal controls we re recruited. We studied the morphology, phenotype, and functional abilities of bone marrow-derived dendritic cells (BMDCs) generated by using two culture methods: FMS-like tyrosine kinase 3 (Flt3)-ligand (FL) and granulocyte-macrophage colony-stimulating factor (GM-CSF) plus interleukin-4 (IL-4), respectively. Results: BMDCs induced by FL exhibited both myeloid (mDC) and plasmacytoid DC (pDC) features, whereas GM-CSF/ IL-4 induced mDC generation. Substantial phenotypic and functional defects of BMDCs were found from patients with SLE at different stages of cell maturation. When compared with healthy controls, SLE immature BM FLDCs expressed higher levels of CCR7. Both immature and mature SLE BM FLDCs expressed higher levels of CD40 and CD86 and induced stronger T-cell proliferation. SLE BM mDCs expressed higher levels of CD40 and CD86 but lower levels of HLA- DR and a lower ability to stimulate T-cell proliferation when compared with control BM mDCs. Conclu