patterns of sequence polymorphism in the fleshless berry locus in cultivated and wild vitis vinifera accessions模式消瘦的浆果的序列多态性位点在栽培和野生葡萄登记入册.pdfVIP

Houel et al. BMC Plant Biology 2010, 10:284 /1471-2229/10/284 RESEARCH ARTICLE Open Access Patterns of sequence polymorphism in the fleshless berry locus in cultivated and wild Vitis vinifera accessions 1* 1,2 3 1 4 4 Cléa Houel , Rémi Bounon , Jamila Chaïb , Cécile Guichard , Jean-Pierre Péros , Roberto Bacilieri , Alexis Dereeper4 1 4 4 2 , Aurélie Canaguier , Thierry Lacombe , Amidou N’Diaye , Marie-Christine Le Paslier , 5,6 5 2 4 4 Marie-Stéphanie Vernerey , Olivier Coriton , Dominique Brunel , Patrice This , Laurent Torregrosa , Anne-Françoise Adam-Blondon1* Abstract Background: Unlike in tomato, little is known about the genetic and molecular control of fleshy fruit development of perennial fruit trees like grapevine (Vitis vinifera L.). Here we present the study of the sequence polymorphism in a 1 Mb grapevine genome region at the top of chromosome 18 carrying the fleshless berry mutation (flb) in order, first to identify SNP markers closely linked to the gene and second to search for possible signatures of domestication. Results: In total, 62 regions (17 SSR, 3 SNP, 1 CAPS and 41 re-sequenced gene fragments) were scanned for polymorphism along a 3.4 Mb interval (85,127-3,506,060 bp) at the top of the chromosome 18, in both V. vinifera cv. Chardonnay and a genotype carrying the flb mutation, V. vinifera cv. Ugni Blanc mutant. A nearly complete homozygosity in Ugni Blanc (wild and mutant forms) and an expected high level of heterozygosity in Chardonnay were r