physicochemical characteristics and immunomodulatory activities of three polysaccharide-protein complexes of longan pulp三个polysaccharide-protein复合物的理化特征和免疫调节活动桂圆肉.pdfVIP

Molecules 2011, 16, 6148-6164; doi:10.3390/molecule OPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Physicochemical Characteristics and Immunomodulatory Activities of Three Polysaccharide-Protein Complexes of Longan Pulp Yang Yi 1,2, Sen Tai Liao 1, Ming Wei Zhang 1,*, John Shi 3, Rui Fen Zhang 1, Yuan Yuan Deng 1 1 and Zhen Cheng Wei 1 Ministry of Agriculture/Bio-tech Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510610, China 2 College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China 3 Guleph Food Research Center, Agriculture and Agri-Food Canada, 93 Stone Road West, Guelph, ON N1G5C9, Canada * Author to whom correspondence should be addressed; E-Mail: mwzhh@. Received: 26 May 2011; in revised form: 27 June 2011 / Accepted: 14 July 2011 / Published: 21 July 2011 Abstract: Three polysaccharide-protein complexes of longan pulp (LP1-3) were isolated in this work. Their physicochemical characteristics and immunomodulatory effects on splenocytes, natural killer (NK) cells and macrophages in vitro were investigated. The carbohydrate portions of LP1-3 were principally composed of glucose, arabinose and mannose. LP3 displayed the maximal moisture absorption, and the thermal stability of LP2 was obviously higher than that of LP1 and LP3. All of them showed the characteristic polysaccharide and protein bands in the Fourier Transform Infrared (FTIR