performance of eudragit coated whispering gallery mode resonator-based immunosensorseudragit涂层的性能回音廊模式resonator-based免疫传感器.pdf

Sensors 2012, 12, 14604-14611; doi:10.3390/s121114604 OPEN ACCESS sensors ISSN 1424-8220 /journal/sensors Article Performance of Eudragit Coated Whispering Gallery Mode Resonator-Based Immunosensors Ambra Giannetti 1, Simone Berneschi 1,2, Francesco Baldini 1, Franco Cosi 1, Gualtiero Nunzi Conti 1 and Silvia Soria 1,* 1 Istituto di Fisica Applicata Nello Carrara (IFAC CNR), Via Madonna del Piano 10, 50019 Sesto Fiorentino, Firenze, Italy; E-Mails: a.giannetti@r.it (A.G.); s.berneschi@r.it (S.B.); f.baldini@r.it (F.B.); f.cosi@r.it (F.C.); g.nunziconti@r.it (G.N.C.) 2 Centro Studi e Ricerche e Museo Storico della Fisica Enrico Fermi, Piazza del Viminale 1, 00184 Roma, Italy * Author to whom correspondence should be addressed; E-Mail: s.soria@r.it; Tel.: +39-055-522-6391; Fax: +39-055-522-6319. Received: 26 September 2012; in revised form: 24 October 2012 / Accepted: 25 October 2012 / Published: 30 October 2012 Abstract: Whispering gallery mode resonators (WGMR) are an efficient tool for the realization of optical biosensors. A high Q factor preservation is a crucial requirement for good biosensor performances. In this work we present an Eudragit®L100 coated microspherical WGMR as an efficient immunosensor. The developed resonator was morphologically characterized using fluorescence microscopy. The functionalization process was tuned to preserve the high Q factor of the resonator. The protein binding assay w