phytochelatin modified electrode surface as a sensitive heavy- metal ion biosensor决定液泡植物螯合肽修饰电极表面敏感的沉重,金属离子的生物传感器.pdfVIP

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phytochelatin modified electrode surface as a sensitive heavy- metal ion biosensor决定液泡植物螯合肽修饰电极表面敏感的沉重,金属离子的生物传感器.pdf

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phytochelatin modified electrode surface as a sensitive heavy- metal ion biosensor决定液泡植物螯合肽修饰电极表面敏感的沉重,金属离子的生物传感器

Sensors 2005, 5, 70-84 sensors ISSN 1424-8220 © 2005 by MDPI /sensors Phytochelatin Modified Electrode Surface as a Sensitive Heavy- Metal Ion Biosensor Vojtech Adam 1,4, Josef Zehnalek 1, Jitka Petrlova 1, David Potesil 1,4, Bernd Sures 2, 3 5 6 1* Libuse Trnkova , Frantisek Jelen , Jan Vitecek and Rene Kizek 1 Department of Chemistry and Biochemistry and 6 Botany and Plant Physiology, Mendel University of Agriculture and Forestry, Zemedelska 1, 611 37 Brno, Czech Republic 2 Univ. Karlsruhe, Zool. Inst. Okol Parasitol 1, Karlsruhe, D-76128 Germany 3 Department of Theoretical and Physical Chemistry and 4Department of Analytical Chemistry, Masaryk University Faculty of Science, Kotlarska 2, 611 37 Brno 5 Laboratory of Biophysical Chemistry and Molecular Oncology, Institute of Biophysics, Academy of Sciences of the Czech Republic, Kralovopolska 135, Brno 612 65, Czech Republic * Author to whom correspondence should be addressed. Email: kizek@sci.muni.cz Received: 06 September 2004 / Accepted: 18 November 2004 / Published: 28 February 2005 Abstract: Electrochemical biosensors have superior properties over other existing measurement systems because they can provide rapid, simple and low-cost on-field determination of many biological active species and a number of dangerous pollutants. In our work, we suggested a new heavy metal biosensor based on interaction of heavy metal ions (Cd2+ and Zn2+) with phytochelatin, which wa