piezoelectric biosensor for a simple serological diagnosis of tularemia in infected european brown hares (lepus europaeus)压电生物传感器的一个简单的血清学诊断感染兔热病的欧洲棕色野兔(天兔座europaeus).pdfVIP
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piezoelectric biosensor for a simple serological diagnosis of tularemia in infected european brown hares (lepus europaeus)压电生物传感器的一个简单的血清学诊断感染兔热病的欧洲棕色野兔(天兔座europaeus)
Sensors 2007, 7, 2825-2834
sensors
ISSN 1424-8220
© 2007 by MDPI
/sensors
Full Research Paper
Piezoelectric Biosensor for a Simple Serological Diagnosis
of Tularemia in Infected European Brown Hares (Lepus
europaeus)
Miroslav Pohanka 1,*, František Treml 2, Martin Hubálek 3, Hana Banďouchová 4 ,
Miroslava Beklová 4 and Jiří Pikula 4
1 Center of Biological Defense, Těchonín, Czech Republic. E-mail: rau@atlas.cz
2 Department of Infectious Diseases and Epizootiology, University of Veterinary and Pharmaceutical
Sciences Brno, Czech Republic. E-mail: tremlf@vfu.cz
3 Institute of Molecular Pathology, University of Defence, Hradec Králové, Czech Republic. E-mail:
hubalek@pmfk.cz
4 Department of Veterinary Ecology and Environmental Protection, University of Veterinary and
Pharmaceutical Sciences Brno, Czech Republic. E-mail: pikulaj@vfu.cz
* Author to whom correspondence should be addressed. E-mail: rau@atlas.cz
Received: 17 October 2007 / Accepted: 14 November 2007 / Published: 19 November 2007
Abstract: Piezoelectric biosensor was used for diagnosis of infection by Francisella
tularensis subsp. holarctica in European brown hares. Two kinds of experiments were
performed in this study. First, sera from experimentally infected European brown hares
(Lepus europaeus) were assayed by piezoelectric biosensor and the seventh day post
infection was found as the first one when statistically significant diagnosis of tularemia was
possible; all other sera collected from hares later
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