planar amperometric glucose sensor based on glucose oxidase immobilized by chitosan film on prussian blue layer平面测量电流的葡萄糖传感器基于葡萄糖氧化酶固定在普鲁士蓝壳聚糖膜层.pdfVIP

Sensors 2002, 2, 127-136 sensors ISSN 1424-8220 © 2002 by MDPI /sensors Planar Amperometric Glucose Sensor Based on Glucose Oxidase Immobilized by Chitosan Film on Prussian Blue Layer 1 1 1 2 2 2 Jianzhong Zhu , Ziqiang Zhu , Zongsheng Lai , Rong Wang , Xiaoming Guo , Xiaqin Wu , Guoxiong Zhang2 2 3 4 , Zongrang Zhang , Yiting Wang and Zongyou Chen 1 IT college, East China Normal University, Shanghai 200062, China 2 Shanghai Teachers University, Shanghai 2000234 3 Shanghai Institute for Medicine Control, Shanghai 200233 4 Department of Surgery, Huashan Hospital, Fudan University, Shanghai 200040 Received: 27 February 2002 / Accepted: 19 March 2002 / Published: 23 April 2002 Abstract: A planar amperometric glucose microsensor based on glucose oxidase immobilized by chitosan film on Prussian Blue layer has been developed. The experimental results show that the optimum detection potential is 50 mV (versus Ag/AgCl) and the optimum pH is 6.5. Under the selective conditions the sensor exhibits excellent sensitivity of 98 nA/M and a linear range of 0.1-6.0 mM. The apparent Michanelis-Menten constant of the sensor is 21 mM. The response time is less than 60 seconds. No apparent change in the response to glucose was observed during one month. Foremost, the int