power for mapping quantitative trait loci in crosses between outbred lines in pigs权力远系繁殖之间的映射在十字架数量性状在猪身上.pdfVIP

Original article Power for mapping quantitative trait loci in crosses between outbred lines in pigs Luis Gomez-Raya* Erling Sehested Department of Animal Science, Agricultural University of Norway, Box 5025, N-1432 As, Norway (Received 28 May 1998; accepted 12 May 1999) Abstract - Power for mapping quantitative trait loci using crosses between segregat- ing populations was studied in pigs. Crossing generates gametic disequilibrium and increases heterozygosity. The condition for a heterozygosity among Fl individuals to be greater than in either line at crossing is that allele frequency should be lower than 1/2 in one line and higher than 1/2 in the other line. Maximum expected power and expected risk were used to compare hierarchical backcross (each boar mated to several sows; contrast within boars), hierarchical intercross (each boar mated to several sows; contrast within both boars and sows) and traditional intercross (each boar mated to one sow; contrast within both boars and sows). The use of hierarchical designs (back- cross and increased for traits with low or intermediate heritabilities. intercross) power For small QTL effects and low heritabilities the hierarchical backcross design gave the highest expected power but also the highest risk. There is not a general design which allocates resources in an optimum fashion across situations (heritabilities, QTL ef- A between with and low risk fect, heterozygosity). compromise designs high power is suggested. A hierarchical backcross design of 400 piglets and heterozygosity 0.68 requires between four (maximumexpected power) and eight boars (minimumrisk) to