prevalence study of bovine viral diarrhea virus by evaluation of antigen capture elisa and rt-pcr assay in bovine, ovine, caprine, buffalo and camel aborted fetuses in iran患病率的研究评估牛病毒性腹泻病毒的抗原捕获elisa和rt - pcr检测牛、绵羊的,山羊的,水牛和骆驼流产胎儿在伊朗.pdfVIP

Safarpoor Dehkordi AMB Express 2011, 1:32 /content/1/1/32 ORIGINAL Open Access Prevalence study of Bovine viral diarrhea virus by evaluation of antigen capture ELISA and RT-PCR assay in Bovine, Ovine, Caprine, Buffalo and Camel aborted fetuses in Iran Farhad Safarpoor Dehkordi Abstract Bovine viral diarrhea virus is a pestivirus in the family Flaviviridae that cause abortions and stillbirths in livestock and its traditional diagnosis is based on cell culture and virus neutralization test. In this study, for more sensitive, specific detection and determined the prevalence of virus in aborted Bovine, Ovine, Caprine, Buffalo and Camel fetuses the antigen capture ELISA and RT-PCR were recommended. From the total of 2173 aborted fetuses, 347 (15.96%) and 402 (18.49%) were positive for presence of Bovine viral diarrhea virus by antigen capture ELISA and RT-PCR respectively. Statistical analysis of data showed significant differences between ELISA and RT-PCR for detection of virus in aborted fetuses. These results indicate a high presence of this pathogen in Iran and that RT- PCR is considerably faster and more accurate than ELISA for identification of Bovine viral diarrhea virus. To our knowledge the Camels and Bovine are the most resistant and sensitive to Bovine viral diarrhea’s abortions respectively and the prevalence of virus in Caprine is more than Ovine aborted fetuses. This study is the first prevalence report of Bovine viral diarrhea virus in aborted Bovine, Ovine, Caprine, Buffalo and Camel fetuses by evaluation of ELISA and RT-PCR in Iran. Keywords: Prevalence study, Bovine viral diarrhea virus, antigen capture ELISA, RT-PCR, aborted fetuses, Iran Introduction syndrome (Coetzer and Tustin 2004; Passler et al. 2007; Bovine viral diarrhea viru