radiolabelling of antigen and liposomes for vaccine biodistribution studiesradiolabelling抗原和疫苗biodistribution研究脂质体.pdfVIP

Pharmaceutics 2010, 2, 91–104; doi:10.3390/pharmaceutics2020091 OPEN ACCESS pharmaceutics ISSN 1999-4923 /journal/pharmaceutics Article Radiolabelling of Antigen and Liposomes for Vaccine Biodistribution Studies Malou Henriksen-Lacey, Vincent Bramwell and Yvonne Perrie * School of Life and Health Sciences, Aston University, Aston Triangle, Birmingham, B4 7ET, UK * Author to whom correspondence should be addressed; E-Mail: y.perrie@aston.ac.uk; Tel.:+ 0121 204 3991. Received: 19 March 2010; in revised form: 29 March 2010 / Accepted: 30 March 2010 / Published: 31 March 2010 Abstract: A relatively simple and effective method to follow the movement of pharmaceutical preparations such as vaccines in biodistribution studies is to radiolabel the components. Whilst single radiolabelling is common practice, in vaccine systems containing adjuvants the ability to follow both the adjuvant and the antigen is favourable. To this end, we have devised a dual-radiolabelling method whereby the adjuvant (liposomes) is labelled with 3H and the antigen (a subunit protein) with 125I. This model is stable and reproducible; we have shown release of the radiolabels to be negligible over periods of up to 1 week in foetal calf serum at 37 ºC. In this paper we describe the techniques which enable the radiolabelling of various components, assessing stability and processing of samples which all for th