原创力文档relative expression of genes of terpene metabolism in different tissues of artemisia annua l萜烯代谢的相关基因的表达在不同组织的青蒿l.pdfVIP
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relative expression of genes of terpene metabolism in different tissues of artemisia annua l萜烯代谢的相关基因的表达在不同组织的青蒿l
Olofsson et al. BMC Plant Biology 2011, 11:45
/1471-2229/11/45
RESEARCH ARTICLE Open Access
Relative expression of genes of terpene
metabolism in different tissues of Artemisia
annua L
*
Linda Olofsson, Alexander Engström, Anneli Lundgren, Peter E Brodelius
Abstract
Background: Recently, Artemisia annua L. (annual or sweet wormwood) has received increasing attention due to
the fact that the plant produces the sesquiterpenoid endoperoxide artemisinin, which today is widely used for
treatment of malaria. The plant produces relatively small amounts of artemisinin and a worldwide shortage of the
drug has led to intense research in order to increase the yield of artemisinin. In order to improve our
understanding of terpene metabolism in the plant and to evaluate the competition for precursors, which may
influence the yield of artemisinin, we have used qPCR to estimate the expression of 14 genes of terpene
metabolism in different tissues.
Results: The four genes of the artemisinin biosynthetic pathway (amorpha-4,11-diene synthase, amorphadiene-12-
hydroxylase, artemisinic aldehyde Δ 11(13) reductase and aldehyde dehydrogenase 1) showed remarkably higher
expression (between ~40- to ~500-fold) in flower buds and young leaves compared to other tissues (old leaves,
stems, roots, hairy root cultures). Further, dihydroartemisinic aldehyde reductase showed a very high expression
only in hairy root cultures. Germacrene A and caryophyllene synthase were mostly expressed in young leaves and
flower buds while epi-cedrol synthase was highly expressed in old leaves. 3-Hydroxy-3-methyl-glutaryl coenzyme A
reductase exhibited lower expression in old leaves compared to other tissues. Farnesyldiphosphate synthase,
squalene synthase, and 1-deoxy-D-
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