remodeling lipid metabolism and improving insulin responsiveness in human primary myotubes改造脂质代谢、改善胰岛素反应在人类主要肌管.pdfVIP

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remodeling lipid metabolism and improving insulin responsiveness in human primary myotubes改造脂质代谢、改善胰岛素反应在人类主要肌管.pdf

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remodeling lipid metabolism and improving insulin responsiveness in human primary myotubes改造脂质代谢、改善胰岛素反应在人类主要肌管

Remodeling Lipid Metabolism and Improving Insulin Responsiveness in Human Primary Myotubes 1.¤a 1.¤b 2 1 3 Lauren M. Sparks , Cedric Moro , Barbara Ukropcova , Sudip Bajpeyi , Anthony E. Civitarese , 4 5 5 1 ¤c Matthew W. Hulver , G. Hege Thoresen , Arild C. Rustan , Steven R. Smith * 1 Molecular and Experimental Endocrinology Laboratory, Pennington Biomedical Research Center, Baton Rouge, Louisiana, United States of America, 2 Diabetes Laboratory, Institute of Experimental Endocrinology, Bratislava, Slovak Republic, 3 Department of Metabolic Physiology, Arizona State University, Phoenix, Arizona, United States of America, 4 Department of Human Nutrition, Food and Exercise, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, United States of America, 5 Department of Pharmaceutical Biosciences, School of Pharmacy, University of Oslo, Oslo, Norway Abstract Objective: Disturbances in lipid metabolism are strongly associated with insulin resistance and type 2 diabetes (T2D). We hypothesized that activation of cAMP/PKA and calcium signaling pathways in cultured human myotubes would provide further insight into regulation of lipid storage, lipolysis, lipid oxidation and insulin responsiveness. Methods: Human myoblasts were isolated from vastus lateralis, purified, cultured and differentiated into myotubes. All cells were incubated with palmitate during differentiation. Treatment cells were pulsed 1 hour each day with forskolin and ionomycin (PFI) during the final