renal expression and serum levels of high mobility group box 1 protein in lupus nephritis肾高机动组框1的表达和血清蛋白在狼疮肾炎.pdfVIP

Zickert et al. Arthritis Research Therapy 2012, 14:R36 /content/14/1/R36 RESEARCH ARTICLE Open Access Renal expression and serum levels of high mobility group box 1 protein in lupus nephritis 1* 2 3 4 4 5 Agneta Zickert , Karin Palmblad , Birgitta Sundelin , Sangeeta Chavan , Kevin J Tracey , Annette Bruchfeld and Iva Gunnarsson1 Abstract Introduction: High mobility group box 1 protein (HMGB1) is a nuclear DNA binding protein acting as a pro- inflammatory mediator following extracellular release. HMGB1 has been increasingly recognized as a pathogenic mediator in several inflammatory diseases. Elevated serum levels of HMGB1 have been detected in autoimmune diseases including Systemic lupus erythematosus (SLE). However, the local expression of HMGB1 in active lupus nephritis (LN) is not known. Here we aimed to study both tissue expression and serum levels of HMGB1 in LN patients with active disease and after induction therapy. Methods: Thirty-five patients with active LN were included. Renal biopsies were performed at baseline and after standard induction therapy; corticosteroids combined with immunosuppressive drugs. The biopsies were evaluated according to the World Health Organization (WHO) classification and renal disease activity was estimated using the British Isles lupus assessment group (BILAG) index. Serum levels of HMGB1 were analysed by western blot. HMGB1 expression in renal tissue was assessed by immunohistochemistry at baseline and follow-up biopsies in 25 patients. Results: Baseline biopsies showed WHO class III, IV or V and all patients had high renal disease activity (BILAG A/B). Follow-up biopsies showed WHO I to II (n = 14), III (n = 6), IV (n = 3) or V