regulation of transcriptome, translation, and proteome in response to environmental stress in fission yeast调节转录组、翻译和裂殖酵母蛋白质组以响应环境压力.pdfVIP

Lackner et al. Genome Biology 2012, 13:R25 /content/pdf/gb-2012-13-4-r25.pdf RESEARCH Open Access Regulation of transcriptome, translation, and proteome in response to environmental stress in fission yeast 1,3 2 2 2 1* Daniel H Lackner , Michael W Schmidt , Shuangding Wu , Dieter A Wolf and Jürg Bähler Abstract Background: Gene expression is controlled globally and at multiple levels in response to environmental stress, but the relationships among these dynamic regulatory changes are not clear. Here we analyzed global regulation during different stress conditions in fission yeast, Schizosaccharomyces pombe, combining dynamic genome-wide data on mRNA, translation, and protein profiles. Results: We observed a strong overall concordance between changes in mRNAs and co-directional changes in translation, for both induced and repressed genes, in response to three conditions: oxidative stress, heat shock, and DNA damage. However, approximately 200 genes each under oxidative and heat stress conditions showed discordant regulation with respect to mRNA and translation profiles, with genes and patterns of regulation being stress-specific. For oxidative stress, we also measured dynamic profiles for 2,147 proteins, comprising 43% of the proteome. The mRNAs induced during oxidative stress strongly correlated with increased protein expression, while repressed mRNAs did not relate to the corresponding protein profiles. Overall changes in relative protein expression correlated better with changes in mRNA expression than with changes in translational efficiency. Conclusions: These data highlight a global coordination and fine-tuning of gene regulation during stress that mostly acts in the sam