resistance evolution to bt crops predispersal mating of european corn borers耐药性进化bt作物predispersal欧洲玉米螟虫的交配.pdfVIP

PLoS BIOLOGY Resistance Evolution to Bt Crops: Predispersal Mating of European Corn Borers 1 2* 1,2 ´ ´ 2 1 Ambroise Dalecky , Sergine Ponsard , Richard I. Bailey , Celine Pelissier , Denis Bourguet 1 Institut National de la Recherche Agronomique (INRA), UMR Centre de Biologie et de Gestion des Populations (CBGP), Campus International de Baillarguet, Montferrier-sur- ´ ´ Lez, France, 2 Laboratoire Dynamique de la Biodiversite, CNRS-Universite P. Sabatier–Toulouse III, Toulouse, France Over the past decade, the high-dose refuge (HDR) strategy, aimed at delaying the evolution of pest resistance to Bacillus thuringiensis (Bt) toxins produced by transgenic crops, became mandatory in the United States and is being discussed for Europe. However, precopulatory dispersal and the mating rate between resident and immigrant individuals, two features influencing the efficiency of this strategy, have seldom been quantified in pests targeted by these toxins. We combined mark-recapture and biogeochemical marking over three breeding seasons to quantify these features directly in natural populations of Ostrinia nubilalis, a major lepidopteran corn pest. At the local scale, resident females mated regardless of males having dispersed beforehand or not, as assumed in the HDR strategy. Accordingly, 0–67% of resident females mating before dispersal did so with resident males, this percentage depending on the local proportion of resident males (0% to 67.2%). However, resident males rarely mated with immigrant females (which mostly arrived mated), the