relevant phosphoproteomic and mass spectrometry approaches useful in clinical research有关phosphoproteomic和质谱分析方法用于临床研究.pdfVIP

López et al. Clinical and Translational Medicine 2012, 1:2 /content/1/1/2 SHORT REPORT Open Access Relevant phosphoproteomic and mass spectrometry: approaches useful in clinical research 1,2* 2 2 1,3* Elena López , Sarbelio Rodríguez Muñoz , Juan López Pascual and Luis Madero Abstract Background: “It’s not what we do, it’s the way that we do it”. Never has this maxim been truer in proteomics than now. Mass Spectrometry-based proteomics/phosphoproteomics tools are critical to understand the structure and dynamics (spatial and temporal) of signalling that engages and migrates through the entire proteome. Approaches such as affinity purification followed by Mass Spectrometry (MS) have been used to elucidate relevant biological questions disease vs. health. Thousands of proteins interact via physical and chemical association. Moreover, certain proteins can covalently modify other proteins post-translationally. These post-translational modifications (PTMs) ultimately give rise to the emergent functions of cells in sequence, space and time. Findings: Understanding the functions of phosphorylated proteins thus requires one to study proteomes as linked- systems rather than collections of individual protein molecules. Indeed, the interacting proteome or protein- network knowledge has recently received much attention, as network-systems (signalling pathways) are effective snapshots in time, of the proteome as a whole. MS approaches are clearly essential, in spite of the difficulties of some low abundance proteins for future clinical advances. Conclusion: Clinical proteomics-MS has come a long way in the past decade in terms of technology/platform development, protein chemistry, and together with bioinformati