原创力文档response surface methodology to optimize enzymatic preparation of deapio-platycodin d and platycodin d from radix platycodi响应面方法优化酶法制备桔梗的deapio-platycodin d和platycodin d.pdfVIP
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response surface methodology to optimize enzymatic preparation of deapio-platycodin d and platycodin d from radix platycodi响应面方法优化酶法制备桔梗的deapio-platycodin d和platycodin d
Int. J. Mol. Sci. 2012, 13, 4089-4100; doi:10.3390/ijm
OPEN ACCESS
International Journal of
Molecular Sciences
ISSN 1422-0067
/journal/ijms
Article
Response Surface Methodology to Optimize Enzymatic
Preparation of Deapio-Platycodin D and Platycodin D from
Radix Platycodi
Wei Li 1,†, Li-Chun Zhao 2,†, Zi Wang 1, Yi-Nan Zheng 1, Jian Liang 2,* and Hui Wang 3,*
1 College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China;
E-Mails: liwei7727@126.com (W.L.); wangzi8020@126.com (Z.W.);
zhenyinan@ (Y.-N.Z.)
2 The Affiliated Ruikang Hospital of Guangxi Traditional Chinese Medical College,
Nanning 530011, China; E-Mail: hyzlc@126.com
3 China-Japan Union Hospital, Jilin University, Changchun 130033, China
† These authors contributed equally to this work.
* Authors to whom correspondence should be addressed; E-Mails: ljruikang@126.com (J.L.);
hwangjlu@126.com (H.W.); Tel.: +86-771-2188589 (J.L.); +86-0431(H.W.).
Received: 3 February 2012; in revised form: 8 March 2012 / Accepted: 19 March 2012 /
Published: 28 March 2012
Abstract: In the present work, we reported the enzymatic preparation of deapio-platycodin
D (dPD) and platycodin D (PD) optimized by response surface methodology (RSM) from
Radix Platycodi. During investigation of the hydrolysis of crude platycosides by various
glycoside hydrolases, snailase showed a strong ability to transform deapio-platycoside
E (dPE) and platycoside E (PE) into dPD and
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