rfx1 regulates cd70 and cd11a expression in lupus t cells by recruiting the histone methyltransferase suv39h1rfx1调节cd70和cd11a表达狼疮t细胞通过招聘组蛋白甲基转移酶suv39h1.pdfVIP

Zhao et al. Arthritis Research Therapy 2010, 12:R227 /content/12/6/R227 RESEARCH ARTICLE Open Access RFX1 regulates CD70 and CD11a expression in lupus T cells by recruiting the histone methyltransferase SUV39H1 * Ming Zhao, Xiaoyan Wu, Qing Zhang, Shuangyan Luo, Gongping Liang, Yuwen Su, Yixin Tan, Qianjin Lu Abstract Introduction: Regulatory factor X-box 1 (RFX1) can interact with DNA methyltransferase 1 (DNMT1) and histone deacetylase 1 (HDAC1), and RFX1 down-regulation contributes to DNA hypomethylation and histone H3 hyperacetylation at the cluster of differentiation (CD) 11a and CD70 promoters in CD4+ T cells of patients with systemic lupus erythematosus (SLE). This leads to CD11a and CD70 overexpression, thereby triggering autoimmune responses. In order to provide more insight into the epigenetic mechanisms leading to the deregulation of autoimmune-related genes in SLE, we asked whether RFX1 is involved in regulating histone 3 lysine 9 (H3K9) tri- methylation at the CD11a and CD70 promoters in SLE CD4+ T cells. Methods: CD4+ T cell samples were isolated from 15 SLE patients and 15 healthy controls. H3K9 tri-methylation levels were measured by chromatin immunoprecipitation (ChIP) and real-time quantitative PCR. CD4+ T cells were transfected with plasmids using the Human T cell Nucleofector Kit. RFX1 and histone methyltransferase suppressor of variegation 3-9 (Drosophila) homolog 1 (SUV39H1) interaction was determined by co-immunoprecipation (co-IP) and Western blot and immunofluorescence staining. CD11a and CD70 mRNA levels were measured by real-time RT-PCR. Results: H3K9 tri-methylation levels were significantly reduced within the CD11a and CD70 promoter regions in SLE CD4+ T