roles of salivary components in streptococcus mutans colonization in a new animal model using nodscid.e2f1？？ mice唾液中的组件变形链球菌的角色使用nodscid殖民在一个新的动物模型。.pdfVIP

Roles of Salivary Components in Streptococcus mutans Colonization in a New Animal Model Using NOD/SCID.e2f12/ 2 Mice 1,2 1 2 Tatsuro Ito , Takahide Maeda , Hidenobu Senpuku * 1 Department of Pediatric Dentistry, Nihon University Graduate School of Dentistry at Matsudo, Chiba, Japan, 2 Department of Bacteriology, National Institute of Infectious Diseases, Tokyo, Japan Abstract Streptococcus mutans plays an important role in biofilm formation on the tooth surface and is the primary causative agent of dental caries. The binding of S. mutans to the salivary pellicle is of considerable etiologic significance and is important in biofilm development. Recently, we produced NOD/SCID.e2f12/ 2 mice that show hyposalivation, lower salivary antibody, and an extended life span compared to the parent strain: NOD.e2f12/ 2. In this study we used NOD/SCID.e2f12/ 2 4 or 6 mice to determine the roles of several salivary components in S. mutans colonization in vivo. S. mutans colonization in NOD/ SCID.e2f12/ 2 mice was significantly increased when mice were pre-treated with human saliva or commercial salivary components. Interestingly, pre-treatment with secretory IgA (sIgA) at physiological concentrations promoted significant colonization of S. mutans compared with sIgA at higher concentrations, or with human saliva or other components. Our data suggest the principal effects of specific sIgA on S. mutans occur during S. mutans colonization, where the appropriate concentration of specific sIgA may serve as an anti-microbial agent, agglutinin, or an adherence receptor to surface antigens. Further, specific sIgA supported biofilm formation when the mice were supplied 1% sucrose water and a non- sucrose diet. The data suggests th