rv2607 from mycobacterium tuberculosis is a pyridoxine 5′-phosphate oxidase with unusual substrate specificityrv2607结核分枝杆菌是一种吡哆醇5u2032磷酸氧化酶与不寻常的底物特异性.pdfVIP

Rv2607 from Mycobacterium tuberculosis Is a Pyridoxine 59-Phosphate Oxidase with Unusual Substrate Specificity 1,2 1 ´ ´2 ´2 1 Ellene H. Mashalidis , Tathagata Mukherjee , Paweł Sledz , Dijana Matak-Vinkovic , Helena Boshoff , Chris Abell2*, Clifton E. Barry III1* 1 National Institute of Allergy and Infectious Disease, National Institutes of Health, Bethesda, Maryland, United States of America, 2 Department of Chemistry, University of Cambridge, Cambridge, United Kingdom Abstract Despite intensive effort, the majority of the annotated Mycobacterium tuberculosis genome consists of genes encoding proteins of unknown or poorly understood function. For example, there are seven conserved hypothetical proteins annotated as homologs of pyridoxine 59-phosphate oxidase (PNPOx), an enzyme that oxidizes pyridoxine 59-phosphate (PNP) or pyridoxamine 59-phosphate (PMP) to form pyridoxal 59-phosphate (PLP). We have characterized the function of Rv2607 from Mycobacterium tuberculosis H37Rv and shown that it encodes a PNPOx that oxidizes PNP to PLP. The kcat and KM for this reaction were 0.01 s21 and 360 mM, respectively. Unlike many PNPOx enzymes, Rv2607 does not recognize PMP as a substrate. ´ ´ ´ Citation: Mashalidis EH, Mukherjee T, Sledz P, Matak-Vinkovic D, Boshoff H, et al. (2011) Rv2607 from Mycobacterium tuberculosis Is a Pyridoxine 59-Phosphate Oxidase with Unusual Substrate Specificity. PLoS ONE 6(11): e27643. doi:10.1371/journal.pone.0027643 Editor: Anil Kumar Tyagi, University of Delhi, India Received Jul