海地瓜和冰岛刺参海参岩藻聚糖硫酸酯抗肿瘤作用的比较研究.pdf

※营养卫生 食品科学 2012, Vol. 33, No. 07 251 张 珣，王静凤，徐 雷，王亚斐，柳 东，薛长湖，李兆杰 * ( 中国海洋大学食品科学与工程学院，山东 青岛 266003) 目的：采用离子交换层析法分别从海地瓜和冰岛刺参中，分离纯化两种海参岩藻聚糖硫酸酯(SC-FUC) ， 通过建立小鼠 Lewis 肺癌自发性肺转移模型，比较分析两种 SC-FUC 对小鼠肿瘤生长和转移的抑制作用。方法：采 用 1- 苯基 -3- 甲基-5- 吡唑啉酮(PMP)柱前衍生高效液相色谱法(HPLC)测定其单糖组成，采用离子色谱法测定其硫酸 基含量。通过连续腹腔注射两种 SC-FUC 21d ，剥离原位肿瘤和双侧肺，分别称瘤质量和计数肺表面转移灶结节 数。采用 RT-PCR 法检测肿瘤组织中缺氧诱导因子(HIF-1 α)和血管内皮生长因子( VEGF) mRNA 的表达。结果：海 地瓜 SC-FUC 和冰岛刺参 SC-FUC 均由岩藻糖组成，硫酸基含量分别为 26.3% 和 29.31% ；两者均能显著抑制荷瘤小 鼠原位肿瘤的生长，抑瘤率分别为19.24% 和 28.69% ；显著减少肺表面转移灶结节数(P ＜0.01) ，转移抑制率分别 为 31.4% 和 57.1% ；显著降低荷瘤小鼠肿瘤组织中HIF-1 α和 VEGF mRNA 的表达(P ＜0.05，P ＜0.01) 。冰岛刺参 SC-FUC 的效果要优于海地瓜SC-FUC 。结论：两种SC-FUC 均能显著抑制肿瘤的生长和转移，其作用机制与其高 硫酸化程度有关。 海地瓜；冰岛刺参；海参岩藻聚糖硫酸酯；Lewis 肺癌；抑瘤率；转移抑制率；HIF-1 α；VEGF Comparative Anti-tumor Effects of Fucoidan from Two Sea Cucumber Species on Spontaneous Metastasis of Lewis Lung Carcinoma in Mouse ZHANG Xun ，WANG Jing-feng ，XU Lei ，WANG Ya-fei ，LIU Dong ，XUE Chang-hu ，LI Zhao-jie* (College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China) Abstract：Purpose: To investigate the influence of fucoidan (SC-FUC) from Cucumaria frondosa and Acaudina molpadioidea on the growth and metastasis of Lewis lung carcinoma in mice. Methods: Lewis lung carcinoma models of C57BL/6J mice were established. SC-FUC was administrated to mice by intraperitoneal injection for 21 consecutive days. Mice were then sacrificed and the subcutaneous tumors were weighed to calculate inhibitory rate. The metastatic tumor foci on lung surface were counted to calculate occurrence rate and inhibitory rate of metastases. The expression of HIF-1α and VEGF mRNA was detected by RT-