study of the extraction process and in vivo inhibitory effect of ganoderma triterpenes in oral mucosa cancer研究灵芝三萜的提取过程和体内抑制作用在口腔黏膜癌.pdfVIP

Molecules 2011, 16, 5315-5332; doi:10.3390/molecule OPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Study of the Extraction Process and In Vivo Inhibitory Effect of Ganoderma Triterpenes in Oral Mucosa Cancer 1 1 1 1 1 2 Yang Gao , Ruhui Zhang , Juan Zhang , Shang Gao , Wenxin Gao , Haifeng Zhang , Haotian Wang 2 and Bing Han 2,* 1 Stomatology Hospital, Jilin University, Changchun 130021, Jilin, China 2 School of Pharmacy, Jilin University, Changchun 130021, Jilin, China * Author to whom correspondence should be addressed; E-Mail: hanb@; Tel.: +86-431 Fax: +86-431 Received: 9 May 2011; in revised form: 3 June 2011 / Accepted: 13 June 2011 / Published: 24 June 2011 Abstract: The aim of the reported study was to optimize the extraction process for ganoderma triterpenes and to investigate the in vivo inhibitory effect of ganoderma triterpenes on the genesis and progression of oral cancer. Single-factor and orthogonal methods were used to investigate the effects of extraction solvent, solvent amount, extraction time, extraction temperature, and number of extractions, on the extraction rate for ganoderma triterpenes. A golden hamster model with cheek pouch dynamic canceration was established to receive oral treatment of ganoderma triterpenes water solution. Animals were