supercritical fluid extraction (sfe) of monoterpenes from the leaves of melaleuca alternifolia (tea tree)超临界流体萃取的单萜白千层属灌木的叶子alternifolia(茶树).pdfVIP
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supercritical fluid extraction (sfe) of monoterpenes from the leaves of melaleuca alternifolia (tea tree)超临界流体萃取的单萜白千层属灌木的叶子alternifolia(茶树)
Molecules 2001, 6, 92-103
molecules
ISSN 1420-3049
© 2001 by MDPI
Supercritical Fluid Extraction (SFE) of Monoterpenes from the
Leaves of Melaleuca alternifolia (Tea Tree)
Victor Wong*, S. Grant Wyllie, Charles P. Cornwell and Deidre Tronson
Centre for Biostructural and Biomolecular Research, Building K12, University of Western Sydney,
Hawkesbury Campus, Locked Bag 1797, Penrith South, NSW 1797, Australia.
Tel.: (02) 4570-1640, Fax: (02) 4570-1621.
* Author to whom correspondence should be addressed. E-mail: v.wong@.au
Received: 23 November 2000 / Accepted: 30 November 2000 / Published: 16 January 2001
Abstract: The technique of supercritical fluid extraction (SFE) was applied to various
sample matrices under a range of supercritical carbon dioxide (scCO ) densities and
2
chamber temperatures. The purpose was to develop an effective extraction condition for the
removal of eight target monoterpenes from Australian tea tree (Melaleuca alternifolia
Cheel) leaves. The optimum conditions for extraction were found to be 0.25 g/mL scCO2
o
density at a chamber temperature of 110 C. These condition were most effective when
applied to whole fresh and rehydrated whole dried leaves, where it yielded maximum
recovery of target analytes with minimum change in oil compositi
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