原创力文档supersage analysis of the nicotiana attenuata transcriptome after fatty acid-amino acid elicitation (fac) identification of early mediators of insect responsessupersage分析烟草attenuata则转录组后脂肪acid-amino酸引出(fac)昆虫的早期介质响应的识别.pdfVIP
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supersage analysis of the nicotiana attenuata transcriptome after fatty acid-amino acid elicitation (fac) identification of early mediators of insect responsessupersage分析烟草attenuata则转录组后脂肪acid-amino酸引出(fac)昆虫的早期介质响应的识别
Gilardoni et al. BMC Plant Biology 2010, 10:66
/1471-2229/10/66
RESEARCH ARTICLE Open Access
SuperSAGE analysis of the Nicotiana attenuata
transcriptome after fatty acid-amino acid
elicitation (FAC): identification of early mediators
of insect responses
1 1 2 2 1 1*
Paola A Gilardoni , Stefan Schuck , Ruth Jüngling , Björn Rotter , Ian T Baldwin , Gustavo Bonaventure
Abstract
Background: Plants trigger and tailor defense responses after perception of the oral secretions (OS) of attacking
specialist lepidopteran larvae. Fatty acid-amino acid conjugates (FACs) in the OS of the Manduca sexta larvae are
necessary and sufficient to elicit the herbivory-specific responses in Nicotiana attenuata, an annual wild tobacco
species. How FACs are perceived and activate signal transduction mechanisms is unknown.
Results: We used SuperSAGE combined with 454 sequencing to quantify the early transcriptional changes elicited
by the FAC N-linolenoyl-glutamic acid (18:3-Glu) and virus induced gene silencing (VIGS) to examine the function
of candidate genes in the M. sexta-N. attenuata interaction. The analysis targeted mRNAs encoding regulatory
components: rare transcripts with very rapid FAC-elicited kinetics (increases within 60 and declines within 120 min).
From 12,744 unique Tag sequences identified (UniTags), 430 and 117 were significantly up- and down-regulated ≥
2.5-fold, respectively, after 18:3-Glu elicitation compared to wounding. Based on gene ontology classification, more
than 25% of the annotated UniTags corresponded to putative regulatory components, including 30 transcriptional
regulators and 22 protein kinases. Quantitative PCR analysis was used to analyze the FAC-dependent regulation of
a subset of 27 of thes
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