streptococcus iniae m-like protein contributes to virulence in fish and is a target for live attenuated vaccine development链球菌iniae m-like蛋白质有助于在鱼毒性,是减活疫苗的目标发展.pdfVIP

Streptococcus iniae M-Like Protein Contributes to Virulence in Fish and Is a Target for Live Attenuated Vaccine Development 1,2 3 4 1,2,5 1,6 Jeffrey B. Locke , Ramy K. Aziz , Mike R. Vicknair , Victor Nizet , John T. Buchanan * 1 Department of Pediatrics, University of California San Diego, La Jolla, California, United States of America, 2 Center for Marine Biotechnology Biomedicine, Scripps Institution of Oceanography, University of California San Diego, La Jolla, California, United States of America, 3 Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo, Egypt, 4 Kent SeaTech Corporation, San Diego, California, United States of America, 5 Skaggs School of Pharmacy Pharmaceutical Sciences, University of California San Diego, La Jolla, California, United States of America, 6 Aqua Bounty Technologies, San Diego, California, United States of America Abstract Background: Streptococcus iniae is a significant pathogen in finfish aquaculture, though knowledge of virulence determinants is lacking. Through pyrosequencing of the S. iniae genome we have identified two gene homologues to classical surface-anchored streptococcal virulence factors: M-like protein (simA) and C5a peptidase (scpI). Methodology/Principal Findings: S. iniae possesses a Mga-like locus containing simA and a divergently transcribed putative mga-like regulatory gene, mgx. In contrast to the Mga locus of group A Streptococcus (GAS, S. pyogenes), scpI is located distally in the chromosome. Comparative sequence analysis of the Mgx locus revealed only one significant variant, a strain with an insertion frameshift mutation in simA and a deletion mutation in a region down