synergistic disruptions in seuss cyp85a2 double mutants reveal a role for brassinolide synthesis during gynoecium and ovule development协同中断苏斯cyp85a2双突变体揭示油菜素内酯合成的作用在雌蕊和胚珠发育.pdfVIP

Nole-Wilson et al. BMC Plant Biology 2010, 10:198 /1471-2229/10/198 RESEARCH ARTICLE Open Access Synergistic disruptions in seuss cyp85A2 double mutants reveal a role for brassinolide synthesis during gynoecium and ovule development † † * Staci Nole-Wilson , Elizabeth E Rueschhoff , Huda Bhatti, Robert G Franks Abstract Background: The Arabidopsis SEUSS (SEU) gene encodes a transcriptional adaptor protein that is required for a diverse set of developmental events, including floral organ identity specification, as well as gynoecium, ovule and embryo development. In order to better understand the molecular mechanisms of SEUSS action we undertook a genetic modifier screen to identify seuss-modifier (sum) mutations. Results: Screening of M2 lines representing approximately 5,000 M1 individuals identified mutations that enhance the seuss mutant phenotypic disruptions in ovules and gynoecia; here we describe the phenotype of the sum63 mutant and enhanced disruptions of ovule and gynoecial development in the seu sum63 double mutant. Mapping and genetic complementation tests indicate that sum63 is allelic to CYP85A2 (AT3G30180) a cytochrome p450 enzyme that catalyzes the final steps in the synthesis of the phytohormone brassinolide. Conclusions: Our identification of mutations in CYP85A2 as enhancers of the seuss mutant phenotype suggests a previously unrecognized role for brassinolide synthesis in gynoecial and ovule outer integument development. The work also suggests that seuss mutants may be more sensitive to the loss or reduction of brassinolide synthesis than are wild type plants. Background this model SEU is required for repression of AG in floral SEUSS (SEU) is a member o