原创力文档template directed reversible photochemical ligation of oligodeoxynucleotides模板直接可逆光化学oligodeoxynucleotides结扎.pdfVIP
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template directed reversible photochemical ligation of oligodeoxynucleotides模板直接可逆光化学oligodeoxynucleotides结扎
Molecules 2012, 17, 163-178; doi:10.3390/molecule
OPEN ACCESS
molecules
ISSN 1420-3049
/journal/molecules
Article
Template Directed Reversible Photochemical Ligation of
Oligodeoxynucleotides
Shigetaka Nakamura 1, Shinzi Ogasawara 1, Shigeo Matuda 2, Isao Saito 2 and Kenzo Fujimoto 1,3,*
1 School of Materials Science, Japan Advanced Institute of Science and Technology, Nomi,
Ishikawa 923-1292, Japan
2 Department of Synthetic Chemistry and Biological Chemistry, Factly of Enginnering,
Kyoto University, Kyoto 606-8501, Japan
3 Research Center for Bio-Architecture, Japan Advanced Institute of Science and Technology, Nomi,
Ishikawa 923-1292, Japan
* Author to whom correspondence should be addressed; E-Mail: kenzo@jaist.ac.jp;
Tel.: +81-0761-51-1671; Fax: +81-0761-51-1671.
Received: 29 November 2011; in revised form: 21 December 2011 / Accepted: 22 December 2011 /
Published: 27 December 2011
V
Abstract: We demonstrated that 5-vinyldeoxyuridine ( U) and 5-carboxyvinyldeoxyuridine
(CVU) can be used to photoligate a longer oligonucleotide (ODN) from smaller ODNs on a
template. By performing irradiation at 366 nm, these artificial nucleotides make
photoligated ODNs with high efficiency without any side reactions. Moreover, by
performing irradiation at 312 nm, these photoligated ODNs were reversed to the original
ODN. VU needs to be irradiated 366 nm for 6 h, but CVU needs to be irradiated at 366 nm
for 15 min. Finally, we made a self-assem
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