textile dye removal from wastewater effluents using bioflocculants produced by indigenous bacterial isolates产生的废水除纺织染料废水利用bioflocculants土著细菌隔离.pdfVIP
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textile dye removal from wastewater effluents using bioflocculants produced by indigenous bacterial isolates产生的废水除纺织染料废水利用bioflocculants土著细菌隔离
Molecules 2012, 17, 14260-14274; doi:10.3390/molecules171214260
OPEN ACCESS
molecules
ISSN 1420-3049
/journal/molecules
Article
Textile Dye Removal from Wastewater Effluents Using
Bioflocculants Produced by Indigenous Bacterial Isolates
Simphiwe P. Buthelezi, Ademola O. Olaniran * and Balakrishna Pillay
Department of Microbiology, School of Life Sciences, College of Agriculture, Engineering and Science,
University of KwaZulu-Natal (Westville Campus), Private Bag X54001, Durban 4000,
South Africa; E-Mails: buthelezisim@ukzn.ac.za (S.P.B.); pillayb1@ukzn.ac.za (B.P.)
* Author to whom correspondence should be addressed; E-Mail: olanirana@ukzn.ac.za;
Tel.: +27-31-260-7400; Fax: +27-31-260-7809.
Received: 8 October 2012; in revised form: 13 November 2012 / Accepted: 26 November 2012 /
Published: 30 November 2012
Abstract: Bioflocculant-producing bacteria were isolated from activated sludge of a
wastewater treatment plant located in Durban, South Africa, and identified using standard
biochemical tests as well as the analysis of their 16S rRNA gene sequences. The
bioflocculants produced by these organisms were ethanol precipitated, purified using 2%
(w/v) cetylpyridinium chloride solution and evaluated for removal of wastewater dyes
under different pH, temperature and nutritional conditions. Bioflocculants from these
indigenous bacteria were very effective for decolourizing the different dyes tested in this
study, with a removal rate of up to
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