the arabidopsis pop2-1 mutant reveals the involvement of gaba transaminase in salt stress tolerance拟南芥pop2-1突变揭示了gaba转氨酶参与盐胁迫宽容.pdfVIP

Renault et al. BMC Plant Biology 2010, 10:20 /1471-2229/10/20 RESEARCH ARTICLE Open Access The Arabidopsis pop2-1 mutant reveals the involvement of GABA transaminase in salt stress tolerance 1,2 1,3 2 1 2 1 Hugues Renault , Valérie Roussel , Abdelhak El Amrani , Matthieu Arzel , David Renault , Alain Bouchereau , Carole Deleu1* Abstract Background: GABA (g-aminobutyric acid) is a non protein amino acid that has been reported to accumulate in a number of plant species when subjected to high salinity and many other environmental constraints. However, no experimental data are to date available on the molecular function of GABA and the involvement of its metabolism in salt stress tolerance in higher plants. Here, we investigated the regulation of GABA metabolism in Arabidopsis thaliana at the metabolite, enzymatic activity and gene transcription levels upon NaCl stress. Results: We identified the GABA transaminase (GABA-T), the first step of GABA catabolism, as the most responsive to NaCl. We further performed a functional analysis of the corresponding gene POP2 and demonstrated that the previously isolated loss-of-function pop2-1 mutant was oversensitive to ionic stress but not to osmotic stress suggesting a specific role in salt tolerance. NaCl oversensitivity was not associated with overaccumulation of Na+ - + and Cl but mutant showed a slight decrease in K . To bring insights into POP2 function, a promoter-reporter gene strategy was used and showed that POP2 was mainly expressed in roots under control conditions and was induced in primary root apex and aerial parts of plants in response to NaCl. Additionally, GC-MS- and UPLC-b